| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Café. |
|
Data corrente: |
12/11/2019 |
|
Data da última atualização: |
12/11/2019 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
SANTOS, F. C. dos; ROSA, S. D. V. F. da; COELHO, S. V. B.; CLEMENTE, A. C. S.; FANTAZZINI, T. B.; PEREIRA, C. C.; CAIXETA, F.; FÁVARIS, N. A. B.; BAÚTE, J. L. |
|
Afiliação: |
Hehagro Pesquisa; STTELA DELLYZETE VEIGA F DA ROSA, CNPCa; Universidade Federal de Lavras - UFLA/Laboratório Central de Sementes/DAG; Grupo Fertilágua; Universidade Federal de Lavras - UFLA/Laboratório Central de Sementes/DAG; Universidade Federal de Lavras - UFLA/Laboratório Central de Sementes/DAG; Universidade Federal de Lavras - UFLA/Laboratório Central de Sementes/DAG; Universidade Federal de Lavras - UFLA/Laboratório Central de Sementes/DAG; Universidade Federal de Lavras - UFLA/Laboratório Central de Sementes/DAG. |
|
Título: |
GA3 and ABI3 gene expression in coffee seeds during maturation. |
|
Ano de publicação: |
2019 |
|
Fonte/Imprenta: |
Genetics and Molecular Research, v. 18, n. 3, 2019 |
|
Idioma: |
Inglês |
|
Conteúdo: |
During maturation in seeds, metabolic processes dependent on gene expression are controlled by hormones, including abscisic acid and gibberellin. These hormones determine dormancy or the capacity for germination in seeds and may have different expression levels in the endosperm and embryo of coffee seeds. We quantified gene expression in the biosynthetic pathway of gibberellin and abscisic acid in different parts of Coffea arabica L. seeds in pre and post physiological maturity, in order to better understand the germination mechanisms of this species. Coffee fruits were harvested at green, yellowish-green, cherry, over-ripe and dry stages. For studies of gene expression, intact seeds were used in addition to endosperm and isolated embryos. The RNA from different tissues was extracted and treated with DNAse to synthesize cDNA. Transition levels of CaGA3 (gibberellin) and CaABI3 (abscisic acid) genes were quantified with qRT-PCR, using specific primers for coffee. Intact seeds at each phenological stage were submitted to germination tests, to evaluate the physiological quality of the seeds. Seed harvested at green and dry stages showed lower hysiological quality when compared to the other maturation stages, and germination at the green stage was close to zero. Greatest expression of CaGA3 and CaABI3 occurs in the endosperm of coffee seeds in cherry and over-ripe stages, with better physiological performance. There are differences in CaABI3 and CaGA3 gene expression in embryos and endosperm of the coffee seeds. MenosDuring maturation in seeds, metabolic processes dependent on gene expression are controlled by hormones, including abscisic acid and gibberellin. These hormones determine dormancy or the capacity for germination in seeds and may have different expression levels in the endosperm and embryo of coffee seeds. We quantified gene expression in the biosynthetic pathway of gibberellin and abscisic acid in different parts of Coffea arabica L. seeds in pre and post physiological maturity, in order to better understand the germination mechanisms of this species. Coffee fruits were harvested at green, yellowish-green, cherry, over-ripe and dry stages. For studies of gene expression, intact seeds were used in addition to endosperm and isolated embryos. The RNA from different tissues was extracted and treated with DNAse to synthesize cDNA. Transition levels of CaGA3 (gibberellin) and CaABI3 (abscisic acid) genes were quantified with qRT-PCR, using specific primers for coffee. Intact seeds at each phenological stage were submitted to germination tests, to evaluate the physiological quality of the seeds. Seed harvested at green and dry stages showed lower hysiological quality when compared to the other maturation stages, and germination at the green stage was close to zero. Greatest expression of CaGA3 and CaABI3 occurs in the endosperm of coffee seeds in cherry and over-ripe stages, with better physiological performance. There are differences in CaABI3 and CaGA3 gene expression in embryos ... Mostrar Tudo |
|
Palavras-Chave: |
QRT-PCR. |
|
Thesagro: |
Coffea Arábica. |
|
Thesaurus Nal: |
Abscisic acid; Gibberellic acid. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/204559/1/Ga3-and-ABI3-gene-expression.pdf
|
|
Marc: |
LEADER 02279naa a2200265 a 4500
001 2114204
005 2019-11-12
008 2019 bl uuuu u00u1 u #d
100 1 $aSANTOS, F. C. dos
245 $aGA3 and ABI3 gene expression in coffee seeds during maturation.$h[electronic resource]
260 $c2019
520 $aDuring maturation in seeds, metabolic processes dependent on gene expression are controlled by hormones, including abscisic acid and gibberellin. These hormones determine dormancy or the capacity for germination in seeds and may have different expression levels in the endosperm and embryo of coffee seeds. We quantified gene expression in the biosynthetic pathway of gibberellin and abscisic acid in different parts of Coffea arabica L. seeds in pre and post physiological maturity, in order to better understand the germination mechanisms of this species. Coffee fruits were harvested at green, yellowish-green, cherry, over-ripe and dry stages. For studies of gene expression, intact seeds were used in addition to endosperm and isolated embryos. The RNA from different tissues was extracted and treated with DNAse to synthesize cDNA. Transition levels of CaGA3 (gibberellin) and CaABI3 (abscisic acid) genes were quantified with qRT-PCR, using specific primers for coffee. Intact seeds at each phenological stage were submitted to germination tests, to evaluate the physiological quality of the seeds. Seed harvested at green and dry stages showed lower hysiological quality when compared to the other maturation stages, and germination at the green stage was close to zero. Greatest expression of CaGA3 and CaABI3 occurs in the endosperm of coffee seeds in cherry and over-ripe stages, with better physiological performance. There are differences in CaABI3 and CaGA3 gene expression in embryos and endosperm of the coffee seeds.
650 $aAbscisic acid
650 $aGibberellic acid
650 $aCoffea Arábica
653 $aQRT-PCR
700 1 $aROSA, S. D. V. F. da
700 1 $aCOELHO, S. V. B.
700 1 $aCLEMENTE, A. C. S.
700 1 $aFANTAZZINI, T. B.
700 1 $aPEREIRA, C. C.
700 1 $aCAIXETA, F.
700 1 $aFÁVARIS, N. A. B.
700 1 $aBAÚTE, J. L.
773 $tGenetics and Molecular Research$gv. 18, n. 3, 2019
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Café (CNPCa) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 11 | |
| 2. |  | FIGUEIREDO, A. G.; ROSA, S. D. V. F. da; CLEMENTE, A. C. S.; MALTA, M. R.; VILELA, A. L. Antioxidant enzyme expression and its relationship to coffee beans quality. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 26., 2016, Kunming,Yunnan, China. Proceedings... ASIC - Association for Science and Information on Coffee, 2016. p. 246.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
|    |
| 4. | | FANTAZZINI, T. B.; ROSA, S. D. V. F. da; CLEMENTE, A. C. S.; PEREIRA, C. C.; OLIVEIRA, R. M. Physiological quality of coffee seeds submitted to artificial and natural ageing. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 26., 2016, Kunming,Yunnan, China. Proceedings... ASIC - Association for Science and Information on Coffee, 2016. p. 128.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
| |
| 5. | | FIGUEIREDO, M. A.; ROSA, S. D. V. F. da; CLEMENTE, A. C. S.; RICALDONI, M. A.; CARVALHO, C. P.; FILHO, R. S.; SOUZA, L. N. C. de. Antioxidantes endógenos e exógenos em sementes de Coffea Arabica L. secadas por diferentes métodos. In: CONGRESSO BRASILEIRO DE PESQUISAS CAFEEIRAS, 42., 2016, Serra Negra, SP. Produzir mais café, com economia, só com boa tecnologia: trabalhos apresentados. Varginha: Fundação Procafé, 2016. 466 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
|  |
| 6. | | FIGUEIREDO, M. A.; ROSA, S. D. V. F. da; CLEMENTE, A. C. S.; CARVALHO, C. P.; RICALDONI, M. A.; VILELA, A. L. Cathodic and catalase enzyme protection in coffee seeds uner different cryopreservation protocols. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 26., 2016, Kunming,Yunnan, China. Proceedings... ASIC - Association for Science and Information on Coffee, 2016. p. 124.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
| |
| 7. | | CLEMENTE, A. C. S.; GUIMARÃES, R. M.; MARTINS, D. C.; GOMES, L. A. A.; CAIXETA, F.; REIS, R. G. E.; ROSA, S. D. V. F. da. Expression of genes associated with the biosynthetic pathways of abscisic acid, gibberellin, and ethylene during the germination of lettuce seeds. Genetics and Molecular Research, v. 14, n. 2, p. 4703-4715, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Café. |
| |
| 8. | | FIGUEIREDO, M. A.; ROSA, S. D. V. F. da; CLEMENTE, A. C. S.; COELHO, S. V. B.; GUIMARÃES, G. C.; REIS, L. V. Enzymatic and cathodic water protection during coffee seed drying. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 26., 2016, Kunming,Yunnan, China. Proceedings... ASIC - Association for Science and Information on Coffee, 2016. p. 125.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
| |
| 9. | | ABREU, G. F. de; ROSA, S. D. V. F. da; CIRILLO, M. A.; MALTA, M. R.; CLEMENTE, A. C. S.; BORÉM, F. M. Simultaneous optimization of coffee quality variables during storage. Revista Brasileira de Engenharia Agrícola e Ambiental, Campo Grande, MS, v. 21, n. 1, p. 56-60, 2017| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Café. |
| |
| 10. | | SOUZA, L. N. C.; ROSA, S. D. V. F. da; GRANJA, M. O.; OLIVEIRA, R. M. E.; VEIGA, A. D.; CLEMENTE, A. C. S. Seed physico-chemical characteristics of coffee clones resistant to rust in raw and toast samples. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 26., 2016, Kunming,Yunnan, China. Proceedings... ASIC - Association for Science and Information on Coffee, 2016. p. 118.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
| |
| 11. | | SANTOS, F. C. dos; ROSA, S. D. V. F. da; COELHO, S. V. B.; CLEMENTE, A. C. S.; FANTAZZINI, T. B.; PEREIRA, C. C.; CAIXETA, F.; FÁVARIS, N. A. B.; BAÚTE, J. L. GA3 and ABI3 gene expression in coffee seeds during maturation. Genetics and Molecular Research, v. 18, n. 3, 2019| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Café. |
| |
| Registros recuperados : 11 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
